The phenoxy ethanol is mainly targeted as a preservative, which can be used in multiple personal care and cosmetic items. You will have a complete cabinet full of things that has phenoxy ethanol in them. Chemically speaking, it is also known as glycol ether and a solvent. It is defined as a slightly sticky and oily liquid with a faint rose-like scent. You might have been in regular contact with this chemical and did not even realize when!
Guidelines for Test Material and Treatment
Understanding more about phenoxy ethanol and following its test material is essential. The more you start researching, the better information will come along your way.
Measurements of Physiological Parameters
The Gp or germination percentage mainly examines the physiological effects of phenoxy ethanol. It will also be measured using root length, relative injury rate and weight gain parameters.
1. The data on germination were also properly determined by analyzing germinated and non-germinated bulbs after around 72 hours of application.
2. The relative injury rate and the GP were calculated using the power of Eqs. 1 and 221.
3. Here, the root lengths were mainly measured based on radical with a millimetre ruler and the weights were then measured with precision scales.
4. The weight gain was mainly determined by calculating the differences between the weights, which had already been measured before and after the exposure.
Examination of cytogenetic parameters
For determining the MN, MI and CAs frequency as cytogenetic parameters, the A. cepa root tip will be prepared. For that, the root tips of the bulb were cut around 1 to 2cm in length and washed with water to remove the residues.
1. The samples were then hydrolyzed with the 1 N HCl at 60 degrees C for around 17 minutes.
2. It is taken into consideration after the fixation process in Clarke fixative.
3. Later, the samples get transferred to a cleaner container and further stained using acetocarmine for one night.
4. Using a research microscope, the root tip preparations were then examined for MI, CAs, and MN analysis. It will then be photographed at 500 times magnification.
5. The MN and the CA frequency were then calculated by analyzing the current 1000 cells from every group.
6.MI will then be calculated using the formula presented in Equation 3, with around 10,000 cells counted for every group.
Is phenoxyethanol safe to use in cosmetics?
Deciding if phenoxyethanol safe to use in cosmetics or not is a debated factor. There is always conflicting data about the safety notion around here. Most of the concerns now stem from the recorded incidents of lousy skin reactions and nervous system interaction in infants.
Currently, the FDA approves phenoxyethanol use in cosmetics and as an indirect food additive. It is only deemed safe when applied in concentrations of 1% or even lower than that. So, the cosmetic companies are very stringent with their guidelines for using phenoxyethanol in their products.